DNA and RNA Repair Therapy in a luxurious and peaceful environment

The immune system can be re-activated with the help of DNA and RNA repair therapies. These are proving valuable for people who are already suffering from some cancers and various genetic conditions.

These therapies are offered at Sanctuary Tulum as alternative ways to combat cancer and also as a preventative measure for those who want to repair any damage from their exposure to environmental and chemical agents.

Lifestyle plays a major role in how people age and the body’s own cell regeneration are not always reliable. Replication of cells by the body is often also accompanied by errors called mutations. Specialized enzymes in DNA and RNA therapies respond to different types of cell damage and can help to heal and re-activate damaged cells.

Experience DNA repair combined with our sacred healing protocol

Experience Complete Brain Restoration:

The Holistic Approach

At the Holistic Sanctuary toxic medications are pushed aside in favor of a three-pronged natural approach that seeks to heal the body, spirit, and mind offering complete healing and repair.

Physical well-being, mental health, and spiritual fulfillment ensure that every client leaves Tulum Sanctuary completely energized and relieved from the symptoms that they brought with them, whether for addictions, genetic conditions, mental disorders and other medical conditions.

The Pouyan Method of treatment was created by the founder of Sanctuary Tulum, Johnny Tabai after he himself was left disappointed by the treatments on offer at many rehabilitation centers.

Johnny realized that the correct combination of the holistic, sacred and modern alternative medicines that helped heal him, also offered the ability to heal many more disorders than just addictions.

Now in the 12th year of living a clean and healthy life, he has devoted 9 of these to help over one thousand people to achieve the same.

The Team

A team of 20, highly qualified, staff members and an ER doctor are there to help assist every client in the intimate and non-judgmental environment at Sanctuary Tulum. The intake is always very small to ensure that each person has the undivided attention of the person taking them through the various therapies, always on a one-on-one basis.

The Treatments

DNA and RNA therapy are just one of the specialized treatments offered in the Pouyan Healing Protocol. Depending on the package that the client has chosen, 28 to 90 days of holistic integrative organic therapies will follow. These include daily massages, yoga meditation, and Reiki sessions, various daily IV therapies that include Mitochondria, liver, and DNA and RNA renewal and repair, various stem cell therapies, and specialized vitamin drips. Other therapies on offer include HBOT, Cryotherapy, Deprivation Therapy, Colonics, Growth Factors, Ozone Therapy, and Immune Builders. Daily Dead Sea baths and Carbon Infrared Sauna sessions offer the body complete relaxation for a faster absorption of therapies.

Sacred plant medicine therapies and a healthy plant-based, 100% raw food diet, which includes protein shakes complete the healing cycle.

The spiritual protocols of the sacred plant ceremonies provide a balanced treatment that can’t be matched and includes Spiritual Awakening, Outer Body Experience God Molecule Activation (DMT) ad Chakra Balancing.

Experience DNA repair combined with our sacred healing protocol

More than just a treatment center

The east coast of Mexico offers its breathtaking landscape with a wide variety of outdoor activities. The Sanctuary Tulum provides a luxury center with exclusive hospitality so that patients can embrace a complete spiritual awakening with a healthy body and mind.

Before & After

What is RNA and DNA Repair Therapy?

RNA and DNA repair therapies are two distinct methods of treatment known as nucleic acid therapies. One scientist likened the two therapies to a building plan where DNA editing is similar to reworking the building plan itself while RNA editing is similar to interpreting the existing plan by adding or removing something to make the design work better. DNA repair therapy has been around for some time but it has proven to have technical limitations, which is why scientists are looking at RNA repair therapy as an alternative approach to genetic therapy. RNA therapy has potential to treat many diseases including heart diseases, hemophilia and cancer.

About DNA Repair Therapy

DNA damage includes mismatched bases, methylation, inter-strand DNA crosslinks, intra-strand DNA crosslinks, DNA double strand breaks and protein-DNA adducts. Cells are constantly identifying and repairing such damage in order to survive, using a very important process called the DNA Damage Response or DDR. Cancer can potentially be treated by inhibiting the DDR pathways and proteins that some cancer cells critically depend on for survival. DNA repair may also involve replacement of DNA in patient cells in order to correct genetic problems or to treat cancer.

What DNA Repair Therapy Can Treat

1. DNA Therapy Being Used to Treat Ovarian and Breast Cancer

DNA repair therapy has potential to treat a wide range of tumors without any resistance from cancer cell. Cancer treatments that target DNA repair mechanisms are fairly new.AstraZeneca’s Lynparza (olaparib) was thefirst PARP inhibitor to receive approvalin 2015, as acommercialized treatment for people with ovarian and breast cancers that do not respond to chemotherapy.Two more PARP inhibitors by competing companies were authorized in 2016 and 2017. Since entering the market in 2015, drugs that target poly (ADP-ribose) polymerase (PARP) have significantly changed the clinical management of cancers that harbor BRCA-mutations (BRCAm).

PARP inhibitors take advantage of weaknesses in cancers with DNA repair defects to target tumors while reducing damage to normal tissue. Research on PARP inhibitors continues as other pharmaceutical giants seek to enter the PARP market.

2. DNA Repair Therapy has Potential to Treat Many Types of Cancer

Paris-based Onxeo is conducting an ongoingPhase1 trialto test 6 different doses of their treatment AsiDNA. By November 2018, they had tested 3 doses which proved to be safe. AsiDNA consists of a short strand of DNA connected to a cholesterol molecule. It hyperactivates the molecular mechanisms that cells use to repair DNA in such a way that proteins involved are not available to repair DNA damage in cancer cells. This does not affect healthy cells. Basically, when AsiDNA enters the cells, healthy cells go into sleeping mode and stop dividing. AsiDNA is metabolized fast so healthy cells wait for it to be removed from the cells then they start to divide again. Tumoral cells are not able to stop dividing so their DNA is damaged and the cancer dies.

Onxeo plans to test AsiDNA in combination with other therapies such as chemotherapy or PARP inhibitors or other DNA-damaging agents. According to Onxeo CEO Judith Greciet, while PARP faces resistance, AsiDNA targets a naturally existing mechanism that is needed for tumor cells to survive and therefore tumor cells cannot develop any resistance to it.

Onxeo’s mechanism is unique in that it targets all the pathways of the repairing mechanism, unlike other compounds that target only one pathway. Therefore,AsiDNA is not limited by any specific mutation or molecule present in tumor cells and therefore it has potential to treat many types of tumors. Research at Onxeo is ongoing.

About RNA Repair Therapy

RNA is an active and powerful commander of the cell machinery. There are RNAs that code and organize protein creation, modify how instruction for DNA are transmitted, destroy other RNAs and prevent rearrangement of genomes. In RNA therapy, a specifically engineered strand of RNA is delivered to produce specific functions, or to interact with specific functions,within the cell. By simply fine-tuning the necleobase code, the therapist can adjust the effects of the therapy.For example, by changing the sequence they create a different effect and if it works once, it multiplies. RNA therapy is therefore more targeted and more versatile than conventional treatments.
There are two types of RNA therapy. Antisense RNA therapy, the more common but complicated type of RNA therapy, destroys dysfunctional or harmful proteins in the cell. Then there is mRNA that produces functional proteins. In simple terms RNA therapy is gene editing that can switch the protein-production equipment within certain cells on and off at will. Effectively it does not cause a permanent change in a cell’s structural plan, but it basically changes the implementation of that plan.

What RNA Repair Therapy Can Treat

RNA repair can one day treat diseases of the kidneys, brain, liver and muscles whose cells do not submit to DNA editing such as CRISPR-Cas9.

1. Gene Therapy for Cystic Fibrosis

The cystic fibrosis transmembrane conductance regulator (CFTR) gene was discovered in 1989. The CFTR gene contains instructions for making the CFTR protein. When there is a mutation (changes to the genetic instructions), the production of CFTR protein can be affected. In cases of cystic fibrosis (CF), mutations in the CFTR gene can result in a protein being made incorrectly or no protein being made or insufficient protein being made. Each of these scenarios cause many problems that affect various organs such as production of thick sticky mucus in the intestines, liver, pancreas and lungs, among other symptoms.Since the discovery of CFTR gene, scientists have been trying to find ways to correct mutations in the gene that cause cystic fibrosis.

Through RNA therapy, a new and correct version of the CFTR gene isplaced into the cells in a person’s body. Although the mutant CFTR gene remains in the body, the presence of the correct copy gives cells the ability to make normal CFTR proteins.

According to Arcturus Therapeutics’ preclinical trials, their RNA-based treatment, containing messenger RNA molecules, can be delivered into the lungs or other organ of a person with cystic fibrosis. Findings of their preclinical research in cells and mice,where they used their LUNAR lipid-mediated delivery technology,show evidence of new human mRNAs that yielded higher levels of functional CFTR protein and evidence of efficient mRNA delivery into the lungs’ epithelial cells using LUNAR-CF. They also showed evidence that the LUNAR technology allowed for inhalation or nebulization, protecting mRNA from damage in DF sputum. Arcturus will soon be conducting clinical trials so they are going to accelerate development of an mRNA-based therapy for cystic fibrosis using LUNAR. “Our goal is to develop a transformative therapeutic that has the potential to treat a broad population of cystic fibrosis patients, regardless of mutation type,” said Joseph Payne, Arcturus president and CEO in apress release.

2. RNA Therapy for Spinal Muscular Atrophy and other Diseases

Genetic alterations, called “nonsense mutations” alter DNA sequence and introduce stop codons that prematurely stop gene expression and damage protein production. The mutations are the underlying causes of spinal muscular atrophy (SMA), polycystic kidney disease, Duchenne muscular dystrophy and cystic fibrosis. Scientists have been looking for compounds that can repair nonsense mutations but they met with challenges. Even CRISPR/Cas9 editing presented challenges.

Now scientists have finally come up with a new gene therapy that uses RNA molecules called transfer RNAs (tRNAs) to repair nonsense mutations. Researchers managed to engineer tRNAs to recognize and suppress 3 different stop codons that prematurely stop gene expression and interfere with protein production. In order to be effective, the edited tRNAs must still be familiar to the cells’ machinery that is involved in translation.

A team of scientists decided to investigate the potential of using tRNAs. In an animal study involving tRNAs, published in the journal Nature Communications, treatmentwas able to repair a subset of mutations that cause spinal muscular atrophy (SMA) and other inherited diseases in living muscle tissue. “In total, the data support the possibility that such engineered tRNA satisfy the broad requirement for coverage of disease-causing (stop codons) and thus represent a promising new class of RNA therapeutic agent,” the researchers wrote.

Of special interest is that a number of different laboratories with different expertise were all able to verify the study’s engineered tRNA technology in a variety of contexts. The team of scientists is hopeful that one day this treatment will be used to correct defective genetic sequences in people.

3. RNA Therapy for Blindness Caused by Genetic Mutations

Leber congenital amaurosis (LCA) is one of the most common causes of blindness in children. A small clinical study involving 10 children with CEP290 mutations who were also diagnosed with LCA was carried out by the Scheie Eye Institute at the University of Pennsylvania and published in Nature Medicine. The children received injections of oligonucleotide, a short RNA molecule, inside the eyes. Oligonucleotide was engineered to decrease the mutant CEP290 protein levels in the photoreceptors in order to restore retinal function.Each patient was givenseveral injections into their worst-seeing eye. Three months after the beginning of the treatment, 50% of the participants had improved visual acuity indicated by the ability to read letters or to see the direction of black and white bars. RNA therapy is therefore a potential treatment of the future for genetic mutations that cause blindness.